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PRISM is free and open source software. You can download both the tool and its source code for free from below. Distribution is under the GNU General Public 

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Significance was determined by two-way Anova (treatment vs dendritic distance; GraphPad Prism version 5.0a), followed by Tukey's HSD post hoc analyses (SPSS version 20; IBM).

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Version 0.3 was released in August 2014, version 0.4 in October 2015, version 0.5 in October 2016, and version 0.6 in June 2017. Both Julia 0.7 (a useful release for testing packages, and for knowing how to upgrade them for 1.0) and version…

The doubling times were calculated experimentally using regression analysis in GraphPad Prism. Although there was a high degree of variability in the bacterial loads, animals harboring lifelong persistent MCMV infection had a significantly higher number of bacteria than did the herpesvirus-free, age-matched controls (Fig. Numbers of animals per group were as follows: Tmprss2-KO Tg, n = 3 per time point (male, 1 to 2; female, 1 to 2); WT, n = 3 per time point (male, 2 to 3; female, 0 to 1). Mice were 19 to 25 weeks old. The dependence of PKC activity on Ca2+ or mol % PS was analyzed by a nonlinear least squares fit to a modified Hill equation as described (57) using GraphPad Prism version 6 (GraphPad Software). Mean values for EC50 and maximal effect (Emax) and SE or 95% confidence limits of these values were calculated by nonlinear regression analysis using the equation for a sigmoid concentration–response curve (GraphPad Prism; Version 5.0). The locomotor cell data were analyzed in 15-min time bins using a two-way repeated measures Anova on SPSS (IBM SPSS Statistics 20.0). The food deprivation-stimulated feeding data were analyzed using Student t test (GraphPad Prism). Kinetic constants were determined using the GraphPad Prism 5.0 software (GraphPad Software) by monitoring the absorption decrease of adenosine (Δε276 = −1.4 m m−1 cm−1), inosine (Δε280 = −0.92 m m−1 cm−1), uridine (Δε280 = −1.8 m m−1 cm−1…

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